snake0613
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as the FA siad :trichomonas vaginalis is diagnosised by trophozoites on wet mount,what mean about the "wet mount"?
thanks
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| ssrpk
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i don't know the dtails but this wetmount prostatic or vaginal secretions helps apprecite the motility of pear shaped trophozoites with a typical jerky motion!
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| Ms.Apara
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dry mount- air dry the slide or heat fix it
wet mount - add a drop of saline while preparing a slide
usually done to see motility
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| bactitech
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Our ER sends us vaginal swabs in about 1 ml of normal saline. We put a drop of this on a microscope slide and coverslip it. This is a "wet mount." We look for motile trichomonads. If they aren't moving you can't call them. We also utilize wet mounts on the bench. For example, if we suspect yeast is growing on a plate, we can put a bit of the colony in saline, coverslip it, and look at it under hi-dry (20X works). Yeasts are large and can be seen easily at that power. Saves making a gram stain and is quicker.
A dry mount is an air dried slide of whatever secretion or fluid you would apply to the slide. In the case of trichomonas, you can make a dried slide of vaginal secretions and stain it with a fluorescent dye called "acridine orange." The trich will fluoresce red with green nuclei under the proper wave length of a fluorescent scope (don't know the settings). We do not use that method at my current employer, but did use it at the outpatient lab I previously worked for because of transport time involved in getting a trich prep to the lab. They die pretty quickly. We get our stuff from ER via a tube system so they come over very quickly. The AO method is more labor intensive.
Dry slides can be coverslipped for permanent mounts. All tissue preps in histology are coverslipped before the pathologists read them.
Hope this helps you out.
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| snake0613
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thanks for all ,I have a basic understanding for it after reading
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