step1mom
Forum Senior
Topics: 32
Posts: 64
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#5:A family has a history of genetic disease with an autosomal sominant pattern of inheritance.Dna from the grandfather who is heterozygous for the disease is cloned and used to construct a restriction map of the gene region involved in the disease.He has a restriction site polymorphism at Eco HV site 2(HV 2)which is useful as a marker.His abnormal chromosome lacks the HV 2site .DNA from his affected grandson is dogested with eco HV ,southern blotted and probed with 32 p_cDNA .complementary to exon 2.Which of the following represents the restriction fragment length polymorphism pattern obtained from the affected grandson?
____exon 1______exon 2__exon3_______exon4__exon5_______exon6___exon7
hv1 hv2 hv3 hv4 hv5
_0_____________1kb____________2kb__________3kb__________4kb___________5kb
_______A____________B___________C___________D____________E______________
4.5 __
4.0 __
__ __ __ __
3.0 __
2.5 __
2.0 __
___________________________________________________________________________
a) A
b)B
c)C
d)D
e)E
Why is the answer B?
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| anjushree
Forum Guru
Topics: 64
Posts: 386
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when u consider about abnormal allele -as it lacks HV-2,fragments will be of 0.5,2.5,1,0.5,0.5.
normal allale (A.D disease)-fragments are 0.5,0.5,2,1,0.5,0.5
so affected grand son has both 2 &2.5 fragments
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| msyamp
Forum Fanatic
Topics: 60
Posts: 1,462
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what is this? all scrambed
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| anjushree
Forum Guru
Topics: 64
Posts: 386
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it is a Q from Q BOOK-Q no 49 from 1st section
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