mjl1717
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Can anyone provide 7 unique entities about staph aureus??
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| parmida
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coagulase+
Beta-hemolytic
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| mjl1717
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Yellow pigment
Portein A
TSSt-1
Enterotoxins
Exfoliatins
Cytolysins
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| bactitech
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For the record, some strains of Staph. aureus are nearly white, and some are very yellow. Also, some are very hemolytic, and some are barely hemolytic.
The coagulase test is the definitive test for this organism. Most strains are slide coag positive, but about 10% only give a positive coag with the tube test. After you've seen a few thousand of these :| you begin to be suspicious if the colony morphology looks like Staph. aureus but your slide test is negative. The tube testing takes a bit longer but is still the gold standard.
Some labs are also using a "rapid" test to determine MRSA. We only use it on positive bloods, CSF, or sterile body fluid cultures because it is so expensive to run, and then we batch them once per shift. It is called PBP2 and many larger labs are using it now. If the PBP2 latex is positive, then it is MRSA. It takes about a half hour to do from start to finish.
http://www.hardydiagnostics.com/02clinic.MRSA.htm...
Apparently this lab's reagent can also be used on coagulase negative Staph. also but our lab does not use it in this manner.
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| whitmer
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Mannitol fermenter on MSA
coag +
DNase +
gold colonies
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