phuluong2k
Forum Fanatic
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22 yo woman with one week of abdominal pain, pelvic exam show red, swollen cervix, gram stain revealed negative gram cocci
Which of the following procedures which most likely lead to the correct diagnosis ?
A. CUlture blood on Thayer Martin Agar
B. DNA probe assay of endocervical exudates
C. Serologic test for specific capsular antigen
D. Germ tube test
E. RPR
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| docrd24
Forum Newbie
Topics: 3
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culture on thayer martin
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| phuluong2k
Forum Fanatic
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I pick A too, but the answer is B
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| Sakaki-
Forum Senior
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Culture blood?
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| mjl1717
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Goes to show you -The field does change. Due to Hi tech and ever since the "Simpson trial" PCR and DNA probes and RFLP are all very much in style!
Some of the distractors:
1)RPR of course Syphilis
2)Germ tube involved with Candida
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Smell the coffee! "Is That an Osler move??"
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| bactitech
Forum Elite
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Actually, one of the most performed tests in our laboratory is the DNA amplification assay for GC and Chlamydia. We use the BD Probetec assay, but there are others.
We still have a few doctors that culture for GC vs. using the DNA amp. kits, but they are getting fewer and fewer. Trying to grow Chlamydia in culture is dicey at best, so this methodology is much more sensitive. The GC and chlamydia assays can be performed on one specimen collected in a special device used only for this test. When I left tonight (Sunday evening) there were probably over 100 of these waiting to be done tomorrow. We batch them and don't do them on weekends.
Please note, however, that in abuse cases there needs to be a culture performed in addition to a DNA amplification assay. I know that before this methodology was available, we had to perform two identification methods on any positive GC on a patient below the age of consent, in case it was abuse. Please keep this in mind if you are going to work in an ER or go into pediatrics. I'm not sure about chlamydia needing two methods, but I would suspect that it would. You may want to check on the legal ramifications more officially with your attendings when the time comes. This is what we have been told repeatedly at our lab meetings, in case the ER calls us regarding this type of case.
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Clinical Microbiology since 1974
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