anatomy
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Using reverse transcriptase and mRNA transcribed from the human glucokinase gene , a researcher constructs a single-stranded cDNA. This cDNA will hybridize with which of the following sequences associated with the glucokinase gene ?
(A) An enhancer sequence
(B) An exon sequence from the coding strand
(C) An intron sequence from the template strand
(D) The promoter sequence from the coding strand
(E) The Shine-Dalgarno sequence from the template strand
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| kmp
Forum Elite
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Ans (E) The Shine-Dalgarno sequence from the template strand
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| Alina T
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My ans is (B) An exon sequence from the coding strand
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| gmc
Forum Newbie
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My ans is also (B). The mRNA is a complement of the exon template strand, therefore identical to the exon coding strand. The cDNA is a complement to the mRNA, therefore it will hybridize to the expn coding strand. :shock:
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| anatomy
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answer is "B".why i don't know.
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