MLF
Forum Elite
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I am a little confused, i was under the impression, that a NEGATIVE tetrazolium blue test confirms the diagnosis of CGD along with a low NADPH oxidase, which I found in FA and in kaplan notes, but according to kaplan Qbank, one question said that the tetrazolium test was positive and the answer given was CGD. Any thoughts????
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| bozhenka
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The standard assay for phagocytic oxidase activity is the nitroblue tetrazolium (NBT) test. The colorless compound NBT is reduced to blue formazan by the activity of the phox enzyme system
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| MLF
Forum Elite
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I think i'll go with kaplan. This is what it says. In the nitroblue tetrazolium test, the yellow nitroblue is converted to purple blue if reactive oxygen intermediates are present, and in CGD, they are not so its a negative test that confirms diagnosis.
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| mdwannabe
Forum Guru
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Kaplan is correct.
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