meghana jadhav
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Cloning of a new eukaryotic gene was followed by insertion and
ligation of the gene in an expression vector. The protein
translated from this gene was then studied on a western blot,
and probed with 32P-DNA, yielding a positive result. These
findings eliminate which of the following substances as a
candidate for the likely gene product?
A. CREB
B. Protein kinase A
C. RNA polymerase
D. SP-1
E. Steroid receptor
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megha
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| mjl1717
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My guess steroid receptor
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| vrach
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My choice is Protein Kianse A
If you are probing for a protein using a DNA probe, then the protein should be a DNA binding protein. Protein Kinase A is the only one that doesen't bind to DNA.
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| meghana jadhav
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u r right vrach
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megha
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| mani
Forum Guru
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if the probe yields a +ve result, ,that means that the protein is a DNA binding protein. rite
but in the question the given protein gave +ve result with DNA so it should be a DNA binding prot. isnt it?
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