Anno Domini
Moderator
Topics: 293
Posts: 727
|
Which are the main regulatory enzymes of hexose monophosphate pathway?
|
| neva
|
The answer is:
35 y.o. AA male has a FLU , and took some OTC med, after which he presents in your office w/ jaundice, dark urine and back pain. Unconjugated bilirubin is elevated. Peripheral blood smear- normocytic nonspherocytic anemia, and there are Heinz bodies seen in some RBCs.
Deficiency of which enzyme is most likely related to this episode?
|
| neva
|
Truly sorry, no dark urine. Uconjugated billirubin is not water soluble and therefore is not going to end up in the urine :oops:
|
| rajeev
Forum Junior
Topics: 5
Posts: 62
|
hmm.. yeah , good concept .. only an increase of indirect bilirubin ..intravascular hemololysis .
can anyone tell me the other conditions where there is intravascular hemolysis ? 4 in all
|
| Yulia
Forum Elite
Topics: 19
Posts: 240
|
G-6-PD deficiency, TTP, DIC, paroxysmal nocturnal hemoglobinuria (PNH), hemolytic anemia due to prosthetic cardiac valves... :?:
|
|
|
way to go ...!!!!!!!
also autoimmune hemolytic anaemia .. i think its the cold type .
how do you differentiate ITP from TTP in a peripheral smear ?
|
| Yulia
Forum Elite
Topics: 19
Posts: 240
|
ITP - Thrombocytopenia with megathrombocytes - enlarged immature platelets
TTP - Thrombocytopenia and schistocytes, reticulocytes
As for hemolysis in cold AIHA, I thought that it was extravascular, in a liver mostly... ::very_confused:: I could be wrong though, need to look it up :icon_study:
|
| rajeev
Forum Junior
Topics: 5
Posts: 62
|
bang on target !!!!!!
are you like taking ur exam tomm or something ?
yeah , i always get confused on the warm or cold AIHA .. i think ur right !
|
| Yulia
Forum Elite
Topics: 19
Posts: 240
|
No, in two weeks 
What about yourself?
|
| rajeev
Forum Junior
Topics: 5
Posts: 62
|
ur ready to rock !!!
me .. sept 17th .. still rusty on lotta boichem and physio
|
| Yulia
Forum Elite
Topics: 19
Posts: 240
|
You still got time You'll be just fine, I am sure.
How about pharm? It is my biggest headache... Do you have any questions to share? Kaplan Q-bank has very few ... surprisingly.
|
| rajeev
Forum Junior
Topics: 5
Posts: 62
|
i think ..pharm is pretty k ....beside that freaking ioniszing and protonted chapter..
im having trouble in genetics and physio..
will be happy to VIVA you sometime in pharm ?
|
| Yulia
Forum Elite
Topics: 19
Posts: 240
|
There is a very unfortunate woman lives (or lived) somewhere. Her mother was a carrier for mutant allele of HRTP gene and father was genotypically normal. The woman suffers from Lesch-Nyhan Disease. What genetic phenomenon could be responsible for disease symptoms in female that should have been just another asymptomatic carrier?
|
| rajeev
Forum Junior
Topics: 5
Posts: 62
|
k .. im just gonna take a chance on this one....but is it that unfortunatley the' sweet ' X chromosome was inactivated ..leaving the nasty X chromosome ...'manifesting heterozygotes '
|
| Yulia
Forum Elite
Topics: 19
Posts: 240
|
Yes, she was unfortunate enough to have most of her "good" chromosomes, meaning carrying normal allele, inactivated.
Here's another one, more of a molecular biology:
They are describing the process of eukaryotic gene cloning, some particular gene, and its product is Western blotted and probed with radioactive DNA, which gives a positive result.
Which of the following is least likely to be a product of that gene?
a. CREB
B. steroid receptor
c. RNA polymerase
d. proteine kinase A
E. SP-1
|
| rajeev
Forum Junior
Topics: 5
Posts: 62
|
good qn ..uve got me hangin....d seems like the only one thats not directly involved
but then again creb and protein kinsae are both invld in glucagon ..
|
| rajeev
Forum Junior
Topics: 5
Posts: 62
|
there is a defect in the 'homeobox ' whixh leads to K Wallenburg syndrome ... which gene has the pathology ?
|
| Yulia
Forum Elite
Topics: 19
Posts: 240
|
Rajeev, Protein kinase A does not actually bind to DNA, so you can figure it out from here...
PAX gene mutation - Klein Waardenburg syndrome- bunch of abnormalities...
|
| Yulia
Forum Elite
Topics: 19
Posts: 240
|
You 're right, it is choice d 
|
| rajeev
Forum Junior
Topics: 5
Posts: 62
|
qn .. Yulia is molecular biologist and is deriving mRNA from a tissue expressing a gene to form a cDNA library , her lab is on low supply of an enzyme need ing to carry out the 'recombinant tecnique so she calls her friend rajeev who works at a loacal pharm firm and orders 2 cases of an enzyme .. WHAT ENZYME DID SHE ORDER IN ORDER TO CARRY OUT FORMING A cDNA LIBRABRY ?
|
| rajeev
Forum Junior
Topics: 5
Posts: 62
|
AWESOME ... CAN YOU TELL ME THE DRUG THAT WOULD ALSO COZ SIMMILAR EFFECTS .... CONTRA IN PREGNANCY ???
|
| Yulia
Forum Elite
Topics: 19
Posts: 240
|
Yulia (the molecular biologist one) is going to need some RNA dependent DNA polymerase - AKA reverse transcriptase
The other one - I admit I do not know, and my guess would be methotrexate ::very_confused::
And if am wrong, could you give me some choices please? :oops:
|
| Yulia
Forum Elite
Topics: 19
Posts: 240
|
It is not exactly about biochemistry, I just need your opinion here.
I am going to conduct a large study to assess the course of type 2 DM under the influence of different environmental factors.
I will have 2 large groups, I'll get volunteers from all over the world. First group is going to be a control group, and the second one is going to have all kinds of attention available. I will put them on calorie-salt- restricted diet, they will be getting organic food only, I will make them exercise every day (may be), and I will educate their family and friends about Diabetes. And in two years I will compare the complications of diabetes rates in two groups.
What do you think about my study design?
|
|
|
absolutely right !!!!! yulia the 'molecular bilogist ' would need some RT .
im sorry  , i shoulda worded the Qn better.. what i meant was simm effects to a 'homeodomain screwup ' in pregnanacy .... isotretintoin for 'acne '
so remember b'fore starting a woman in the reproductive age on this drug .. always order a pregnancy test just to make sure shes not pregnant !
|
| rajeev
Forum Junior
Topics: 5
Posts: 62
|
pt has his dna assesed in a folate meduim which shows long arms resembling x .. he has a family h/o of mental retardation and huge testis
where is the pathology located
a. introns
b. 5' UTR
c.3'UTR
d. shine dalgarno sequence
e. promoter
|
|
| |
| | | | | | | | | | | | | | | | | | | | | | | | | |
