bluedusk
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Which organisms are detected by a silver stain?
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| MLF
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PCP is one of them
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| meghana jadhav
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Pneumocystis carini Cryptococcus neoformans.
Coccidoides immitus....r the GMS..STAINED ORGANISMS
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megha
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| mash
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legionella
H pylori
pneumocystis carinii
bartonella hensleae
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I hear and I forget. I see and I remember. I do and I understand.
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| bluedusk
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Cool! H. pylori was the one I couldn't recall, but didn't know about the fungi....
Nice answers....
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| mjl1717
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I agree:
Just want to add that like Neisseria Gonorrhoeae-Legionella is fastidious requiring "special and unique
entities' (cysteine and iron){Buffered Charcaol Yeast} provides this.
Also-DFA for dx, but my main point is when one lecturer said the term Dieterle stain, I didnt realize that Dieterle was a silver stain and the "term" Dieterle has specific ring or intonation to it!
**Neisseria Gonorrhoeae requires high CO2
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| mjl1717
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It certainly doesnt mention the silver stain for the fungi inKaplan or HiYield!
Im not talking about PCP, thats the most popular one!
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Smell the coffee! "Is That an Osler move??"
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| bluedusk
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Yeah, I think we're exploring the "outside" Kaplan/FA world here : )
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| bactitech
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When a "silver stain" is ordered in a clinical laboratory, it usually indicates that the doc wants a fungal stain performed by the Histology/cytology laboratory. Most microbiology departments don't use this stain, as it's fussy. It's also performed on tissues, hence the need to go to histology.
Micro labs usually use calcofluor staining nowadays for fungi. When KOH is ordered, we don't use KOH, we use calcofluor.
BTW, calcofluor is MUCH faster to perform than silver stain, but the lab must have a fluorescent microscope handy. My former boss was doing calcofluors on PCP stains, and they fluoresced quite well. However, one really needs to know what they're looking at.
http://tinyurl.com/5jtjs
(citation at bottom is my former Ph.D. and current one - they did quite a bit of work with calcofluor in the 80's)
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Clinical Microbiology since 1974
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