drrabines
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Single aminoacid substitution
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| BumbleB
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substitution methinkst (d)
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| Malaysian
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I'm not sure if D is right or not since I don't have the chart to check the codons coding for amino acid.But A and B make no sense to me.C makes sense to me as this is frame shift mutation and so if the protein is longer than 4 amino acids most likely it will be truncated......I think the question is a bit vague.
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| BumbleB
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The protein wont be truncated unless you insert a stop codon which are UAA, UAG, UGA
("you are annoying", "you are gross" "you go away") :wink:
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| BumbleB
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Of course that's the rna sequence for the stop codons, for correspoding dna codons just replace the U with a T
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| Malaysian
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Thanks bumble bee...I'm aware of the codons meant for 'stop' the reason why I said the protein is truncated is because I'm assuming the protein to be more than 4amino acid long and frameshift mutations generally lead to truncation(since the question didn't say how many amino acids make up the protein I may be wrong here).If there is a single substitution of an amino acid then the question should have mentioned/hinted that we are suppose to see the chart for converitng the codons into amino acid(and since the question didn't D may be right or wrong).
Have no clue what A and B are talking about....incase if the answer is A or B pls. explain the answer.Bye for now.
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| BumbleB
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Yeah frameshift usually do lead to truncation, but in this case is says that this sequence is near the 3' end - so may be it will lead to a longer mRNA product or some other problem (splicing, processing, etc?). I'm not sure - what's the answer?
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| Malaysian
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Actually this is the 5' end of the RNA because theamino terminus is the first end to get transcribed and this is done by the 5'end of the mRNA.And so you see there is a good chance the protein turns out truncated....however I do agree with you that if this was the carboxy- terminus,truncated protein would have been unlikely....I still have no clue what A and B are implying???
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| BumbleB
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I think A is just a nonsense answer, B could be an answer if it was an obvious promoter that would be affected (TATAA, CCAATT)?
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| Malaysian
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whats TATAA and CCAATT??Is this the TATA box seen in the UTR 5' region?? Do you know any other specific sequences(apart from the start and stop codons)???
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