mani
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1. Klebsiella
2. ....
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| mjl1717
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2)Proteus
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| mash
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H pylori
Ureaplasma urealyticum
there is one fungus too....i dont remember, any one?
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| Ahab
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I think its cryptococcus
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| mdwannabe
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crypto is correct...urase test is one of the quick tests in crypto meningitis, in HIV.
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| dariush
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i thought the quickest way is to check the spinal tap with INDIAN INK for Crypto. menang. (stains the Capsule)
mdwannabe, what is different in AIDS? give me a ref.
thanx.
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| doc_rajan
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Hi guys!
The best way to memorize urease positive organisms is using the pnemonic 'PUNCH'
P-Proteus
U-Ureaplasma
N-Nocardia
C-Cryptococcus
H-Helicobacter
Helicobacter is oxidase positive as well..
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| mani
Forum Guru
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the list is:
Klebsiella
Proteus
Ureaplasma
H pylori
Cryptococcus (fungus)
i cant confirm about nocardia, any input??
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| mdwannabe
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Dariush...i can't remember the source..sorry...
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| dariush
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thanx mdwannabe for replying.
has anyone else heard about preference of Urease test for Crypto menangitis over Indian Ink in HIV ?
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| mani
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no, its not mentioned for crypto any where. what about nocardia?
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| doc_rajan
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All strains of Nocardia are urease positive.. Its written in Jawetz
Only some strains of Klebsiella are urease positive..
There's nothing different in AIDS.. its just that crypto meningitis is more common in AIDS.. Also, India Ink stain is not preferred much for diagnosis as it is not very sensitive (only about 50% positivity). Latex Particle Agglutination Test for Cryptococcal Antigen in serum or CSF is best (90% positivity). I've not read of urease test being used as a rapid method
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| dariush
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thanx doc_rajan
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| bactitech
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Some are, some are not. - oops, see next post.
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| bactitech
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Some are slightly positive, most are not.
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| bactitech
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Cryptococcal antigen testing is the most sensitive. Many hospital labs do not perform India Ink testing anymore and rely exclusively on the antigen.
Our lab had three positive Cryptos this spring; two in CSF's within a week of each other and another in a blood culture from an IV drug user. The latter took four days to come up in the blood culture bottles.
The organisms don't gram stain particularly well on direct smears and can fool some inexperienced micro people (as it did to one of our techs this spring, unfortunately). The bug showed readily in the India Ink prep and looked like a textbook picture. The antigen result was off the charts.
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| dariush
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thanx bactitech for the update 
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