namf
Forum Elite
Topics: 80
Posts: 312
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Hi everyone!
I am so lost on the subject of -1/kM plots, I don't even know where to begin!?
What does kM stand for, anyway!? Why are we making this weird looking fraction!!
I think the chances are good that I will see a question related to where a certain plot crosses the x or y axis, or another line, and I'll have no idea where to begin! Anyone can tell me how to handle these problems/discuss such a question they've seen?
Thanks a lot! ](*,)
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| mjl1717
Forum Hero
Topics: 956
Posts: 5,452
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For starters I can tell you Km is the substrate conc. where you have one half Vmax
On second thought this may be better off in a hard core Biochem forum
with Michaealis Menton and Lineweaver Burke, which many will attempt to ignore. [But their are pharmacokinetic implications]
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Smell the coffee! "Is That an Osler move??"
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| Sakaki-
Forum Senior
Topics: 1
Posts: 238
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The Lineweaver-Burk plot allows us to get a linear plot by plotting 1/V0 vs. 1/[S]. The main advantage is to accurately determine 1/Vmax (y-intercept), which is only approximated using a conventional plot of V0 vs. [S]. -1/Km is the x-intercept of the plot.
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| namf
Forum Elite
Topics: 80
Posts: 312
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Thanks a lot Sasaki- and mjl1717--at least I have a chance now.
Anybody able to post such a question they've seen to illustrate the points?
I know it's hard without the ability to plot a graph..but you could refer to an outside web address :!: 
Thank you very much!
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