Prep for USMLEPrep for USMLE Forum
   Forum    Step 1  Step 2 CK Step 2 CS Step 3  Match  IMGs Resources Search






Previous Topic | Next Topic  tRNA? 




 



Author8 Posts
  #1

A gene product is thought to produce tRNA. What characteristics tells you that is it tRNA?
A)presence of many modified bases
B) 7 methyl guanine cap
C) Poly A tail

  #2

A

___________________
I hear and I forget. I see and I remember. I do and I understand.
--Confucius

  #3

Capping on 5' end (7-methyl-G), polyadenylation on 3' end (~ 200 A's), and splicing out of neutrons occur in the nucleus. Capped and tailed transcripts are mRNA properties.

So A is the answer.
Can someone please explain the "wobble hypothesis" in regards to tRNA?

  #4

The degeneracy/redundancy of the genetic code allows for one amino acid to be encoded by more than one codon. Most of the codons encoding for the same AA only differ in their 3rd codon.
Therefore, according to the Wobble hypothesis, the t-RNA's anticodon only needs to form perfect base pairs with the first two bases on the codon for it to translate the codon and provide the right AA.
The 1st base of the anticodon that pairs with the 3rd base of the codon (codon and anticodon and complementary and anti-parallel) is pretty much irrelevant and so they need not pair. This is called the "Wobble". This allows a single t-RNA to translate several codons that encode for the same AA, and differ only in their 3rd base.
Hope this helps.

Q. Codon CGU is one of four different types of codons for Aregenine. Which of the following changes would not affect the primary structure of protein X, which contins one Argenine group.
A. Sustituting the 'G' of the anti-codon with a 'C'
B. Sustituting the 'A' of the anti-codon with a 'C'
C. Sustituting the 'C' of the anti-codon with a 'G'
D. Sustituting an 'A' for the 'C' on the anti-codon
E. Sustituting an 'A' for the 'G' on the anti-codon

This is a Q that I made up. Let me know if it makes any sense.

  #5

B - probably

  #6

A.

___________________
Allah (God) is watching over us...

  #7

The answer is B.
The t-RNA anticodon is complementary and anti-parallel to the m-RNA codon. The m-RNA codon CGU is written in the 5' to the 3', so the t-RNA anticodon would be ACG (5' to 3'). Since there are 4 types of codons coding for Argenine, we can safely assume that they differ in the 3rd base pair of the codon, then apply the wobble hypothesis.

  #8

sorry i meant to answer Monet`s Q. i think that is A
what is the answer Monet?

___________________
Allah (God) is watching over us...







You don't have permission to post.




Login or Register to post messages in this topic





















Contact | Leaders | Disclaimer | Privacy

Copyright @ Prep for USMLE. All rights reserved.